Magnetic resonance imaging (MRI) combined with contrast agents is believed to be useful for stemcell tracking in vivo, and the aim\nof this research was to investigate the biosafety and neural induction of SD rat-originated adipose derived stem cells (ADSCs) using\ncationic superparamagnetic iron oxide (SPIO) nanoparticlewhich was synthesized by the improved polyol method, in order to allow\nvisualization using in vitro MRI. The scan protocols were performed with T2-mapping sequence; meanwhile, the ultrastructure\nof labeled cells was observed by transmission electron microscopy (TEM) while the iron content was measured by inductively\ncoupled plasma-atomic emission spectrometry (ICP-AES).After neural induction, nestin andNSE (neural markers) were obviously\nexpressed. In vitroMRI showed that the cationic PEG/PEI-modified SPIOnanoparticles could achieve great relaxation performance\nand favourable longevity. And the ICP-AES quantified the lowest iron content that could be detected by MRI as 1.56âË?¼1.8 pg/cell.\nThis study showed that the cationic SPIO could be directly used to label ADSCs, which could then inductively differentiate into\nnerve and be imaged by in vitro MRI, which would exhibit important guiding significance for the further in vivo MRI towards\nanimal models with neurodegenerative disorders
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